Focus: Quantifying cellular migration and mechanical forces exerted by tumor cells using bead tracki...

Bead Tracking and Traction Force Microscopy (TFM)

Focus: Quantifying cellular migration and mechanical forces exerted by tumor cells using bead tracking and TFM techniques.

Key Contributions:

• Implemented advanced image processing techniques to track bead displacement over time, using Particle Image Velocimetry (PIV) and Free-Form Deformation (FFD).

• Optimized algorithms for sub-pixel accuracy in bead tracking, improving the resolution and reliability of traction force calculations.

• Analyzed force distributions at the cell-substrate interface, correlating mechanical outputs with observed cell behaviors.

  
  

  Significance: This work elucidated the mechanical forces exerted by tumor cells, providing a quantitative basis for understanding cell motility and interaction with their microenvironment.